Neugebauer Group

Publications

  • Zhang, E.; Neugebauer, M.E.; Krasnow, N.A.; Liu, D.R.; Phage-assisted evolution of highly active cytosine base editors with enhanced selectivity and minimal sequence context preference. Nat. Commun. 2024, 15, 1697. link
  • Doman, J.L.*; Pandey, S.*; Neugebauer, M.E.; An, M.; Davis, J.R.; Randolph, P.B.; McElroy, A.; Gao, X.D.; Raguram, A.; Richter, M.F.; Everette, K.A.; Banskota, S; Tian, K.; Tao, Y.A.; Tolar, J.; Osborn, M.J.; Liu, D.R. Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell 2023, 186, 18, 3983–4002. link *these authors contributed equally
  • Slater, J.W.; Lin, C-.,Y.; Neugebauer, M.E.; McBride, M.J.; Sil, D.; Nair, M.A.; Katch, B.J.; Boal, A.K.; Chang, M.C.Y.; Silakov, A.; Krebs, C.; Bollinger Jr., J.M. Synergistic binding of the halide and cationic prime substrate of L-lysine 4-chlorinase, BesD, in both ferrous and ferryl states. Biochemistry 2023, 62, 16, 2480–2491. link
  • Kissman, E.N.*; Neugebauer, M.E.*; Sumida, K. H.; Swenson, C.V.; Sambold, N.A.; Marchand, J.A.; Millar D.C.; Chang, M.C.Y. Biocatalytic control of site-selectivity and chain length-selectivity in radical amino acid halogenases. Proc. Natl. Acad. Sci. U.S.A. 2023, 120 (12) e2214512120. link *these authors contributed equally
  • Neugebauer, M.E.; Hsu, A.; Arbab, M.; Krasnow, N.A.; McElroy, A.N.; Pandey, S.; Doman, J.L.; Huang, T.P.; Raguram, A.; Banskota, S.; Newby, G.A.; Tolar, J.; Osborn, M.J.; Liu, D.R. Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target editing. Nat. Biotechnol. 2023, 41, 673–685. link
  • McBride, M.J.; Nair, M.A.; Sil, D.; Slater, J.W.; Neugebauer, M.E.; Chang, M.C.Y.; Boal, A.K.; Krebs, C.; Bollinger Jr., J.M. A Substrate-triggered µ-Peroxodiiron(III) Intermediate in the 4-Chloro-L-Lysine-Fragmenting Heme-Oxygenase-like Diiron Oxidase (HDO) BesC: Substrate Dissociation from, and C4 Targeting by, the Intermediate. Biochemistry 2022, 61, 689–702. link
  • Neugebauer, M.E.; Kissman, E.N.; Marchand, J. A.; Pelton, J.G.; Sambold, N.A; Millar, D.C.; Chang, M.C.Y. Reaction pathway engineering converts a radical hydroxylase into a halogenase. Nat. Chem. Biol. 2022, 18, 171–179. link
  • Neugebauer, M.E.; Sumida, K. H.; Pelton, J.G.; McMurry, J. L.; Marchand, J. A.; Chang, M.C.Y. A family of radical halogenases for the engineering of amino acid-based products. Nat. Chem. Biol. 2019, 15, 1009–1016. link
  • Marchand, J.A.; Neugebauer, M.E.; Ing, M.C.; Lin, C. -I.; Pelton, J.G.; Chang, M.C.Y. Discovery of a pathway for terminal-alkyne amino acid biosynthesis. Nature 2019, 567, 420–424. link